Optimal Parameters of Magnetic Field Action and their Influence on Some Index Improvement in Traumatic Brain Injury Rat Modell

Abstract

Taguchi method

Background and Aim: Traumatic Brain Injury (TBI) remains a significant medical challenge with limited effective treatments. Recent studies suggest that magnetic field therapy can enhance neural recovery. However, the optimal parameters for magnetic field application in TBI models are not well established. The aim of this study was to find out the optimal parameters for applying magnetic field to the recovery of TBI rats by using Taguchi method and to determine the effect of magnetic field on survival rate and some functional index of the TBI rats. Methods: Fourteen albino Wistar male rats weighed 220-250 g were used in the experiment. An experimental model of TBI rats was constructed by Feeney’s method. Optimal parameters of magnetic field was identified by Taguchi orthogonal array. Magnetic field was applied to the experimental group TBI rats for 5 days. Modified Neurological Severity Score (mNSS), Brain Water Content (BWC), electrical stimulation threshold and survival rate were measured. Results: The optimal magnetic field parameters, determined by using one-factor experiment and Taguchi method were magnetic induction at 20 mT, frequency at 20 Hz, duration for 25 min and daily application frequency of 2 times per day. When applied to a TBI rat model, magnetic field of the optimal parameters decreased brain water content and increased survival rate. In addition, modified Neurological Severity Score (mNSS), electrical stimulation threshold were decreased. Conclusion: Optimal parameters of alternating magnetic field for functional recovery of TBI rats improved neuro dysfunction and increased survival rate in TBI rats.