Link of Oxidative stress to Pulmonary Functions Contributed by Matrix Metalloproteinase in South Indian Population-An Observational Study

Abstract

Background and Aim: Oxidative stress plays a key role in the etiopathogenesis of several disease conditions and most commonly pulmonary diseases. Pulmonary tissues are susceptible to oxidative stress from ambient air pollution. Serum Malondialdehyde is a sensitive biomarker of oxidative stress. Oxidants also enhance proteinase mediated lung injury. In the current study we have aimed at correlating the serum Malondialdehyde levels with pulmonary functions and serum Matrix mettaloproteinase-9 levels in south Indian population. Methods: This was a cross sectional study conducted among healthy volunteers of age group 18 to 45 years. Pulmonary function tests of the subjects were recorded using computerised spirometry (SPIROLAB III). Serum malondialdehyde levels were estimated using Thiobarbituric acid Reactive Substances assay and serum Matrix metalloproteinase-9 levels were estimated by Enzyme linked Immunosorbent Assay. Results: Significant negative correlation was found between Malondialdehyde and FEV1/FVC ratio and significant positive correlation was found between serum malondialdehyde levels and Matrix metalloproteinase-9 levels. Individual link of Malondialdehyde levels and FEV1/FVC ratio, malondialdehyde levels and Matrix metalloproteinase-9 levels were established by linear regression analysis. Conclusion: Our results state that increased oxidative stress directly affects pulmonary functions and it also increases MMP-9 levels, which again lead to decline in pulmonary functions.